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Tobacco addiction leads to periods of withdrawal shortly after having a cigarette, whereby the person who smokes experiences psychological symptoms such as low mood, anxiety, poor concentration and irritability (Benowitz, 2010)

Figure 5 4 Discussion 4.1 Physiological response of tobacco leaves to field chilling stress In summary, the physiological, metabolic, and protein-level responses of tobacco leaves under cold stress form a highly coordinated process, with interactions between these layers working together to ensure the plants adaptability to adverse environments

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Rustica Tobacco (Nicotiana rustica) originates from Andean South America, and also has a history of use and cultivation in North America, Vietnam and Russia